DNA Extraction
Aim: to extract DNA from Kiwifruit
Equipment:
- Kiwifruit
- Water
- Beakers
- Sand
- Spatula
- Ethanol
- Sandwich Bags
- Bunsen Burners
- Heat Mat
- Gauze Mat
- Tripod
- Test Tube
- Test Tube Rack
- Dishwash Liquid
- Lighter
- Glass Rod
- Cloth
Method:
- Step 1 Open the Sandwich Bag and put half a Kiwifruit into the bag. Put Sand and Water into the Bag.
- Step 2 Squish the Kiwifruit until there are no chunks left.
- Step 3 Once squished, pour the Kiwifruit through the cloth to collect the juice in the beaker. Then transfer the Kiwifruit into the Test Tube.
- Step 4 Pour 10ml of Water into the Beaker. Then, put the beaker on top of the Tripod and warm the water. (Take it off when you see bubbles forming on the bottom of the beaker)
- Step 5 Pour 2 drops of Dishwashing Liquid into the Test Tube that has the Kiwifruit juice. Then rest the Test Tube in the Beaker with warm water.
- Step 6 Add the Ethanol into the Tes Tube and watch the DNA separate and collect with Spatula.
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